par4 agonist peptide aypgkf-nh2  (GenScript corporation)

Journal: The Journal of Biological Chemistry

Article Title: Mapping Human Protease-activated Receptor 4 (PAR4) Homodimer Interface to Transmembrane Helix 4 *

doi: 10.1074/jbc.M112.341438

Figure Lengend Snippet: TABLE 1

Article Snippet: Cells (2.5 × 10 5 ) were placed into 96-well plates, stimulated with PAR4 agonist peptide (AYPGKF-NH 2 , 1 m m ; Polypeptide Group, Strasbourg, France), and read in a NOVOstar plate reader (BMG Labtech, Durham, NC).

Techniques:

Journal: Blood

Article Title: RGS/G i2α interactions modulate platelet accumulation and thrombus formation at sites of vascular injury

doi: 10.1182/blood-2010-05-283846

Figure Lengend Snippet: Akt phosphorylation and Rap1 activation in Gi2α(+/G184S) platelets. (A) Phosphorylation of Akt after addition of the PAR4 agonist peptide AYPGKF (250μM). Mean ± SEM of data from 3 experiments. One mouse of each genotype was used in each experiment. Samples from the representative pAkt blot were run on the same gel. (B) Rap1 activation in response to varying concentrations of ADP. Mean ± SEM of data from 3 experiments. Two mice of each genotype were used in each experiment. Samples from the representative Rap1 blot were run on the same gel.

Article Snippet: PAR4 agonist peptide H-AYPGKF-NH2 was from Bachem.

Techniques: Activation Assay